I had my one week Hari Raya break and the holidays is going to end very soon.Classes will start from tomorrow =s During this holidays I can say that I spent half of my holiday time is the lab in order to do some experiments.
I went to the lab by volunteer,with some of my course mates.We went there to do bacteria culturing on the CMC agar.CMC is the abbreviation for carboxymethylcellulose. We did this practical with the help from the scientific officer,Mr.Dinesh and also our Bioprocess technology lecturer,Mr.Prabakaran.
Since there is only 3 of us,we had the chance to prepare the agar,all by ourselves!We got the chance to go to the chemical room and take the chemicals,and the do the calculations to obtain the amount of chemicals that we need.Besides,the s.o also taught us how to operate an autoclave!All this is quite impossible when we have practical session during normal times due to the number of students.
We spent an afternoon to prepare our agar which would be use as media later and then the next day,we streak the bacteria onto the media.After two days we went back to the lab again and checking the result.To our surprise,we manage to get a positive result!!Sir said very good and told us to contact the lecturer and tell him about this ^^Sir said we are the only group who get a positive result for CMC agar =D
We are so happy to hear that and feel like we did not waste our time during the last few days!
Below is our results and also some of th epictures that we took in the lab =)
This is the positive result.
The yellow portion shows that cellulose had been digested by the bacillus.
A comparison of positive and negative result.
The results from all the plates.
We used Congo Red and 0.8M Sodium Hydroxide to see our result.
Group photo : Wong,May Chee and I
Me and May Chee
-Very good!-
No comments:
Post a Comment